Recombinant ACE2 SARS-CoV2 Binding Domain

- Overview
  - The sequence below is that of our recombinant construct of the ACE2 extracellular domain which includes the entire region which interacts with both SARS-CoV1 and SARS-CoV2. The specific binding of both SARS viruses to ACE2 is essential for viral internalization and infection. We designed this construct based on amino acids 1-500 in the S-protein sequence in BAB40370.1. This is a defined globular domain recently shown to include all of the amino acids necessary for ACE2 binding (4). Human ACE2 is 805 amino acids long so this construct is missing the C-terminal 305 amino acids, which at 741-763, includes a membrane spanning domain. The construct lacks this region as inclusion would likely have made the molecule difficult to express. The construct was expressed in and purified from E. coli and includes an N-terminal His-tag and other vector derived sequence shown underlined below. Amino acids printed in bold below are those which have been shown to interact with the SARS-CoV2 binding domain.
    
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- Properties
  - Type
    
    Recombinant Proteins
    
    Formulation
    
    Supplied as 1mg/mL in 6M urea, 10mM phosphate buffer pH=7.5
    
    Storage
    
    Stable at 4°C for several months. For longer term store at -20°C, minimize freeze/thaw cycles
    
    More Information
    
    UniProt
    
    Q9BYF1
    
    * For Research Use Only. Not for use in diagnostic/therapeutics procedures.
- Applications
  - Application
    
    ELISA
    
    Application Description
    
    ELISA

Recombinant ACE2 SARS-CoV2 Binding Domain

Recombinant ACE2 SARS-CoV2 Binding Domain

More Information

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Recombinant SARS-CoV2 S-Protein ACE2 Binding Domain