Rapid 26S Proteasome Purification Kit-L

Rapid 26S Proteasome Purification Kit-L

Catalog Number:
PAS1097314UBP
Mfr. No.:
J4320
Price:
$1,013
  • Size:
    1 Kit
    Quantity:
    Add to Cart:
      • Overview
        • The Rapid 26S Proteasome Purification Kit is designed to rapidly purify the endogenous 26S proteasome from cell or tissue extracts within 8 hours according to a published method (reference 1) with modifications. This approach uses the N-terminal ubiquitin-like (Ubl) domain of human RAD23B as an affinity bait, which allows the rapid and gentle isolation of endogenous 26S proteasomes. The bound 26S proteasome is subsequently eluted using the C-terminal two ubiquitin-interacting motifs (UIM) of human S5a.

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      • Properties
        • Formulation
          Enzyme stocked in 20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol; Substrate stocked in DMSO
          Storage
          Store at -80°C; avoid multiple freeze-thaw cycles
          Concentration
          See tube label

          * Research Use Only

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