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Overview
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Immunoproteasome Activity Fluorometric Assay Kit I was designed for assaying the immunoproteasomes' chymotrypsin-like activity in vitro using purified proteasome, cell lysates or tissue extracts. The proteasome cleaves Ac-ANW-AMC and the released AMC fluorescence can be monitored using a plate reader or fluorometer at the excitation/emission wavelength of 360nm/460nm, respectively.
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Overview