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Overview
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Immunoproteasome Activity Fluorometric Assay Kit II was designed for assaying the immunoproteasomes' chymotrypsin-like, trypsin-like and branched amino acid preferring activities in vitro using purified proteasomes, cell lysates or tissue extracts. The proteasome cleaves these fluorogenic substrates, and the released AMC fluorescence can be monitored using a plate reader or fluorometer at the excitation/emission wavelength of 360nm/460nm, respectively.
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Overview