R-Phycoerythrin Labeling Kit-SH

Phycobiliproteins are fluorescent proteins derived from cyanobacteria and eukaryotic algae. Their fluorescence is much higher than chemical fluorescent probes such as fluorescein and rhodamine. R-phycoerythrin (R-PE) is one of the phycobiliproteins and has an orange fluorescence at around 578 nm, and it can be excited at 488 nm. Because of this high fluorescence, phycobiliprotein-labeled antibodies or other molecules can give greater sensitivity in flow cytometry and immunostaining. R-phycoerythrin Labeling Kit-SH is for simple and rapid preparation of R-PE-labeled IgG. SH-reactive R-PE (a component of this kit) has a maleimide group and can easily make a covalent bond with a sulfhydryl group of the target molecule without any activation process. The filtration tube in this kit allows a quick buffer exchange and concentration of sample IgG solution. This kit contains all the reagents necessary for R-PE labeling, including the reducing agent for preparation of reduced IgG that has an SH group and the storage buffer for conjugates.

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