R-Phycoerythrin Labeling Kit-NH2

- Overview
  - Phycobiliproteins are fluorescent proteins derived from cyanobacteria and eukaryotic algae. Their fluorescence is much higher than chemical fluorescent probes such as fluorescein and rhodamine. R-Phycoerythrin (R-PE) is one of the phycobiliproteins and has a red fluorescence at around 578 nm, and it can be excited at 488nm. Because of this high fluorescence, phycobiliprotein labeled antibodies, and other molecules can provide greater sensitivity in flow cytometry and immunostaining. R-phycoerythrin Labeling Kit-NH2 is for simple and rapid preparation of R-PE-labeled IgG. NH2-reactive R-PE (a component of this kit) has an activated ester group and can easily make a covalent bond with an amino group of the target molecule without any activation process. The filtration tube in this kit allows a quick buffer exchange and concentration of sample IgG solution. This kit contains all of the reagents necessary for R-PE labeling, including the Storage buffer for conjugates.
    
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- Properties
  - Categories
    
    Fluorophore labeling
    
    Storage
    
    0-5°C
    
    Shipping
    
    with blue ice
    
    * For research use only
- Applications
  - Application
    
    FCM; Microscopy
- Reference
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    2) K.Y Su, A. Watanabe, C.H. Yeh, G. Kelsoe, M. Kuraoka, "Efficient Culture of Human Naive and Memory B Cells for Use as APCs", J. Immunol.., 2016, 197, (10), 4163.
    
    3) T. Tsujikawa, T. Yaguchi, G. Ohmura, S. Ohta, A. Kobayashi, N. Kawamura, T. Fujita, H. Nakano, T. Shimada, T. Takahashi, R. Nakao, A. Yanagisawa, Y. Hisa, Y. Kawakami, "Autocrine and paracrine loops between cancer cells and macrophages promote lymph node metastasis via CCR4/CCL22 in head and neck squamous cell carcinoma", Int. J. Cancer., 2013, 132, (12), 2755.
    
    4) Y. Inaguma, Y. Akahori, Y. Murayama, K. Shiraishi, S. Tsuzuki-Iba, A. Endoh, J. Tsujikawa, A. Demachi-Okamura, K. Hiramatsu, H. Saji, Y. Yamamoto, N. Yamamoto, Y. Nishimura, T. Takahashi, K. Kuzushima, N. Emi, Y. Akatsuka, "Construction and molecular characterization of a T-cell receptor-like antibody and CAR-T cells specific for minor histocompatibility antigen HA-1H", Gene Ther.., 2014, 21, (6), 575.
    
    5) A. Watanabe, K. R. McCarthy, M. Kuraoka, A. G. Schmidt, Y. Adachi, T. Onodera, K. Tonouchi, T. M.Caradonna, G. Bajic, S. Song, C. E. McGee, G. D. Sempowski, F. Feng, P. Urick, T. B. Kepler, Y. Takahashi, S. C. Harrison, and G. Kelsoe, "Antibodies to a Conserved Influenza Head Interface Epitope Protect by an IgG Subtype-Dependent Mechanism.", Cell.., 2019, 177, (5), 1124.