Allophycocyanin Labeling Kit-SH

Phycobiliproteins are fluorescent proteins derived from cyanobacteria and eukaryotic algae. Their fluorescence is much higher than that of chemical fluorescent probes such as fluorescein and rhodamine. Allophycocyanin (APC) is one of the phycobiliproteins, and it has a red fluorescence at around 660 nm. Because of this high fluorescence, phycobiliprotein-labeled antibodies and other molecules can provide greater sensitivity in flow cytometry and immunostaining. Allophycocyanin Labeling Kit-SH is for simple and rapid preparation of APC-labeled IgG. SH-reactive APC (a component of this kit) has a maleimide group and can easily make a covalent bond with a sulfhydryl group of the target molecule without any activation process. The filtration tube in this kit allows a quick buffer exchange and concentration of sample IgG solution. This kit contains all the reagents necessary for APC labeling, including a reducing agent for preparation of reduced IgG that has an SH group and a storage buffer for conjugates.

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