Allophycocyanin Labeling Kit-NH2

- Overview
  - Phycobiliproteins are fluorescent proteins derived from cyanobacteria and eukaryotic algae. Their fluorescence is much higher than chemical fluorescent probes such as fluorescein and rhodamine. Allophycocyanin (APC) is one of the phycobiliproteins, and it has a red fluorescence at around 660 nm. Because of this high fluorescence, phycobiliprotein-labeled antibodies and other molecules can provide greater sensitivity in flow cytometry and immunostaining. Allophycocyanin Labeling Kit-NH2 is for simple and rapid preparation of APC-labeled IgG. NH2-reactive APC (a component of this kit) has an activated ester group and can easily make a covalent bond with an amino group of the target molecule without any activation process. The filtration tube in this kit is used for buffer exchange and concentration of sample IgG solution. This kit contains all the reagents necessary for APC labeling including the storage buffer for conjugates.
    
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- Properties
  - Categories
    
    Fluorophore labeling
    
    Storage
    
    0-5°C
    
    Shipping
    
    with blue ice
    
    * For research use only
- Applications
  - Application
    
    FCM; Microscopy
- Reference
  - 1) H. Shinohara, T. Yasuda, Y. Aiba, H. Sanjo, M. Hamadate, H. Watarai, H. Sakurai and T. Kurosaki, "PKCβ Regulates BCR-mediated IKK Activatioin by Facilitating the Interaction between TAK1 and CARMA1", J. Exp. Med., 2005, 202, 1423.
    
    2) E. Grace Suto, Y. Mabuchi, N. Suzuki, K. Suzuki, Y. Ogata, M. Taguchi, T. Muneta, I. Sekiya and C. Akazawa, "Prospectively isolated mesenchymal stem/stromal cells are enriched in the CD73+ population and exhibit efficacy after transplantation", Sci. Rep.., 2017,(7), 4838.
    
    3) H. Fujii, Y. Ikeuchi, Y. Kurata, N. Ikeda, U. Bahrudin, P. Li, Y. Nakayama, R. Endo, A. Hasegawa, K. Morikawa, J. Miake, A. Yoshida, K. Hidaka, T. Morisaki, H. Ninomiya, Y. Shirayoshi, K. Yamamoto, I. Hisatome, "Electrophysiological properties of prion-positive cardiac progenitors derived from murine embryonic stem cells", Circ. J.., 2012, 76, (12), 2875.
    
    4) T. Kaya, S. Nagatoishi, K. Nagae, Y. Nakamura and K. Tsumoto , "Highly sensitive biomolecular interaction detection method using optical bound/free separation with grating-coupled surface plasmon field-enhanced fluorescence spectroscopy (GC-SPFS).", PLoS ONE., 2019, 14, (8), DOI:10.1371/journal.pone.0220578.