QuantiChrom™ Fatty Acid Uptake Assay Kit

LONG CHAIN FATTY ACIDS (LCFA) are important fuel sources for animals as substrates in β-oxidation, and serve as building blocks for many different cellular structures. Long-chain unesterified fatty acids (LCFA) are transported into cells using membrane transport proteins, and increased LCFA levels in cells are common in diabetes, obesity-related diseases, cardiovascular disease, and certain forms of cancer. Therefore, fatty acid uptake is a significant therapeutic target for the treatment of metabolic disorders and important topics for metabolic research.Our fluorescent cell-based fatty acid uptake assay uses a fluorescent fatty acid analog which is taken up by fatty acid transporter proteins and accumulates within the cell. Quench reagent is added to block extracellular fluorescent signal in the medium. The adherent cells import the fatty acid analog, and the bottom-read fluorimeter measures the increase in fluorescence signal at λex/em = 488/523nm. This high-throughput assay can be applied to assess fatty acid uptake activity in cells and to screen for activators and inhibitors.

Key Features
▪ Safe. Non-radioactive assay.
▪ Fast and Sensitive. Homogenous “add-and-read” assay. No wash, lysis, or staining steps needed.
▪ Simple and Convenient. Can be automated as a high-throughput assay for fatty acid transport and modulator screens in cells.

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