QuantiChrom™ FRAP Assay Kit

FERRIC REDUCTION ANTIOXIDANT POTENTIAL (FRAP) is a measure of antioxidant capacity quantified by the antioxidant potential to reduce ferric iron (III) to ferrous iron (II). Antioxidants protect cells from damage by reactive oxygen species that are produced by oxidation reactions. As oxidative stress contributes to the development of many diseases including Alzheimer’s disease, Parkinson’s disease, diabetes, rheumatoid arthritis and neurodegeneration, the use of antioxidants in pharmacology is intensively studied. Antioxidants are also widely used as dietary supplements and as preservatives in a wide range of products such as food, cosmetics, rubber and gasoline. Simple, direct and high-throughput assays for antioxidant capacity find wide applications in research, food industry and drug discovery. Our improved assay measures antioxidant potential in which Fe3+ is reduced by antioxidant to Fe2+. The resulting Fe2+ specifically forms a colored complex with a chromogen. The color intensity at 590 nm is proportional to FRAP in the sample.

Key Features
▪ Sensitive and accurate. Linear detection range: 0.5 to 180 µM Fe3+ reduction potential in 96-well plate assay.
▪ Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 40 min. Can be readily automated as a high-throughput assay for thousands of samples per day.
▪ Improved reagent stability and versatility. The optimized formulation has greatly enhanced reagent and signal stability.

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