QuantiChrom™ Detergent Assay Kit

Detergents are surfactants that are amphiphilic, which means that they are partly hydrophobic and partly hydrophilic. Detergents fall into three categories: anionic, cationic, and non-ionic/zwitterionic. They are commonly used in household cleaning products, in gasoline as additives, and in biological reagents. Simple, direct and automation-ready procedures for measuring detergent concentration in biological samples are very desirable.Our detergent assay kit is designed to measure detergent directly without any pretreatment. Above the critical micellar concentration, detergent forms micelle in solution that trap the colorimetric dye. The intensity of the color is directly proportional to the detergent concentration in the sample.

Key Features
▪ Sensitive and accurate. Use 20 µL sample. Linear detection range: Tween 80: 0.012 to 4 mM; Tween 20: 0.06 to 5 mM; Triton X-100: 0.23 to 12 mM; Brij L23/35: 0.09 to 5 mM; DTAC: 0.08 to 2 mM; SDS: 10 to 25 mM.
▪ Simple and convenient. The procedure involves addition of two reagents and measuring OD560nm or OD650nm.
▪ Improved reagent stability and versatility. The optimized formulation has greatly enhanced reagent and signal stability. Cuvette or 96-well plate assay.

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