Name: Puromycin Aminonucleoside
Price: 632.00 USD

- Overview
  - Puromycin Aminonucleoside is the aminonucleoside portion of Puromycin. It is a Puromycin derivative that lacks the methoxyphenylalanyl group on the amine of the sugar ring. The compound is used in animal models of nephrosis and can induce apoptosis. It has antineoplastic properties. Puromycin was isolated from Streptomyces alboniger in the 1950s. Puromycin is routinely used in cell culture as a selective agent in transfection and transformation protocols to select for cells that have been transformed with the pac gene and express puromycin-N-acetyl-transferase. Puromycin Aminonucleoside is soluble in water.
    
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    Background
    
    Puromycin Aminonucleoside interferes with protein synthesis, terminating the ribosomal chain.
- Properties
  - CAS Number
    
    58-60-6
    
    Molecular Formula
    
    C12H18N6O3
    
    Molecular Weight
    
    294.31 g/mol
    
    Appearance
    
    White crystalline powder
    
    Solubility
    
    Soluble in water with heat as needed
    
    Other Properties
    
    Source: Streptomyces Alboniger
    Melting Point: 215-216 °C
    Purity Level: ≥99.0%
    
    Storage
    
    2-8 °C
    
    * For research use only
- Applications
  - Application Description
    
    Spectrum: Gram-positive bacteria, neoplastics, parasites
    
    Eukaryotic Cell Culture Applications: Cells that are resistant to Puromycin express the pac gene which encodes an N-acetyl puromycin transferase. The pac gene can be mobilized on a plasmid and used to transfect a host cell, thus it can be used in mammalian gene selection protocols. A kill curve is performed to determine the minimum effective concentration to kill non-resistant cells. For additional information regarding cell lines, resistance plasmids, and culture media, please visit our Cell Culture Database.
    Puromycin Aminonucleoside can induce apoptosis, thus it can be used to explore the mechanisms of glomerular disease by inducing nephropathy. Glomerular diseases are characterized by podocyte injury or depletion. Podocyte apoptosis is a major factor causing this depletion. Podocytes are specialized epithelial cells in the glomerulus of the kidneys. Nephrin is believed to be the major component of the slit diaphragm, a complex of podocyte-specific proteins that play a role in glomerular filtration. The compound is used to study podocyte biology and quantify the amount of nephrin in a podocyte, to improve our understanding of kidney disease.
    
    Cancer Applications: Puromycin Aminonucleoside is an antimetabolite and antineoplastic agent that is useful in cancer chemotherapy.