Name: Puromycin Dihydrochloride
Price: 219.00 USD

- Overview
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    Background
    
    Puromycin Dihydrochloirde inhibits protein synthesis in two ways: 1) Puromycin associates with the donor substrate, peptidyl-tRNA, in the P site and functions as an acceptor substrate. 2) Puromycin DiHCl can compete with aminoacyl tRNA to bind with the A′ site within the peptidyl transferase center causing premature chain termination.
    Resistance to Puromycin is conferred by the pac gene, a 60 nt fragment that encodes puromycin N-acetyltransferase. The enzyme inactivates Puromycin by acetylating the amino group in the tyrosinyl moiety. Acetylated Puromycin is biologically inactive and does not associate with prokaryotic or eukaryotic ribosomes.
- Properties
  - CAS Number
    
    58-58-2
    
    Molecular Formula
    
    C22H29N7O5 · 2HCl
    
    Molecular Weight
    
    544.43
    
    Appearance
    
    White or off-white powder
    
    Solubility
    
    Water: Freely soluble at pH 7
    
    Storage
    
    2-8°C
    
    * For research use only
- Applications
  - Application Description
    
    Spectrum: Puromycin can prevent growth of bacteria, algae, protozoa, and mammalian cells and acts quickly, killing 99% of cells within 2 days. Puromycin DiHCl is active against both prokaryotic and eurkaryotic cells. It is active against Gram-positive bacteria and less active against Gram-negative and acid-fast bacilli.
    
    Microbiology Applications: Puromycin Dihydrochloride can be used to select for Puromycin resistant bacteria that have been transformed with the pac gene. Resistant E. coli transformants can be isolated on pH adjusted LB medium using a Puromycin concentration of 100-125 µg/mL.
    Puromycin DiHCl can also be used as a selectable marker in mollicute research and has been successfully used to select for various Mycoplasma species after transformation with the Puromycin resistance gene (pac). Tetracycline is traditionally used as a selectable maker for Mycoplasma; however, Puromycin does not have any clinical value, is a potent protein synthesis inhibitor, and can be used to screen For additional information regarding relevant cell lines, resistance plasmids, and culture media, please visit our cell culture database.
    
    Cancer Applications: Puromycin Dihydrochloride has shown anti-tumor activity when tested against numerous cell lines.