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Figure 1. Agonist-induced Threonine362/Serine363 phosphorylation of the Nociceptin/Orphanin FQ Receptor. Upper panel, HEK293 cells stably expressing the Nociceptin/Orphanin FQ receptor (NOP) were either not exposed or exposed to 10 μM Nociceptin/Orphanin FQ or 0.1 μM PMA (Phorbol 12-Myristate 13-Acetate) for 30 minutes. Cells were lysed and immunoblotted with the anti-pT362/pS363-NOP antibody (7TM0320C) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with the phosphorylation-independent anti-NOP antibody (7TM0320N-WB) at a dilution of 1:1000 to confirm equal loading of the gel.

Figure 2. Analysis of dose-dependent Nociceptin/Orphanin FQ Receptor phosphorylation using a panel of phosphosite-specific antibodies. Upper three panels, HEK293 cells stably expressing the Nociceptin/Orphanin FQ Receptor (NOP) were either not exposed or exposed to increasing concentrations of Nociceptin/Orphanin FQ ranging from 1 nM to 10 μM for 30 minutes. Cells were lysed and immunoblotted with the anti-pS346-NOP antibody (7TM0320A) or anti-pS351-NOP antibody (7TM0320B) or anti-pT362/pS363-NOP antibody (7TM0320C) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with the phosphorylation-independent anti-NOP antibody (7TM0320N-WB) at a dilution of 1:1000 to confirm equal loading of the gel.

Figure 3. Analysis of dose-dependent Nociceptin/Orphanin FQ Receptor phosphorylation in vivo in NOP-eGFP knock-in mice using two phosphosite-specific antibodies. Upper two panels, NOP-eGFP knock-in mice stably expressing the Nociceptin/Orphanin FQ Receptor tagged with enhanced green fluorescent protein (NOP-eGFP) were intraperitoneal injected with 0.9% NaCl or increasing concentrations of specific Nociceptin/Orphanin FQ receptor agonist AT-202 ranging from 0.3 to 30 mg/kg for 30 minutes. Mice were euthanized and brains were removed. The NOP-eGFP receptor was immunoprecipitated with anti-GFP protein agarose beads and immunoblotted with the anti-pS351-NOP antibody (7TM0320B) or anti-pT362/pS363-NOP antibody (7TM0320C) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with the phosphorylation-independent anti-NOP antibody (7TM0320N-WB) at a dilution of 1:1000 to confirm equal loading of the gel.

pT362/pS363-NOP ( phospho-Nociceptin/Orphanin FQ Receptor Polyclonal Antibody )

pT362/pS363-NOP ( phospho-Nociceptin/Orphanin FQ Receptor Polyclonal Antibody )

Catalog Number:

A001455978TMA

Mfr. No.:

7TM0320C

Price:

$528

Size:

100 µl

Quantity:

Add to Cart:

Overview
- Threonine362/Serine363 (T362/S363) is a major phosphorylation site of the nociceptin/orphanin FQ peptide receptor (NFQ receptor, NOP). The pT362/pS363-NOP antibody detects phosphorylation in response to agonists but not after PKC activation. T362/S363 phosphorylation is a key regulator of NOP desensitization, β-arrestin recruitment and internalization. The pT362/pS363-NOP antibody can detect phosphorylated MOP in mouse brain in vivo.
  
  Please contact us at for specific academic pricing.
Properties
- Host
  
  rabbit
  
  Antibody Type
  
  polyclonal
  
  Isotype
  
  IgG
  
  Reactivity
  
  human; mouse; rat
  
  Immunogen
  
  A synthetic phosphopeptide derived from human NOP around the phosphorylation site of Thr362/Ser363
  
  Purification
  
  Antigen affinity chromatography
  
  Formulation
  
  Liquid
  
  Storage
  
  Short-term storage at 4°C and long-term storage at -20°C
  
  Antigen
  
  NOP, OPRL1, Nociceptin/Orphanin FQ Receptor (UniProt:P41146 (human), P35377 (mouse), P35370 (rat))
  
  * This product is intended for research use only.

Applications

Application

WB

Application	Dilutions
Western Blot (WB)	1:1000

Reference
- Mann A, Moulédous L, Froment C, O'Neill PR, Dasgupta P, Günther T, Brunori G, Kieffer BL, Toll L, Bruchas MR, Zaveri NT, Schulz S. Agonist-selective NOP receptor phosphorylation correlates in vitro and in vivo and reveals differential post-activation signaling by chemically diverse agonists. Sci Signal. 2019 Mar 26;12(574). pii: eaau8072. doi: 10.1126/scisignal.aau8072. PubMed PMID: 30914485; PubMed Central PMCID: PMC6934085.

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