pT363-KOP ( phospho-κ-Opioid Receptor Polyclonal Antibody )

Overview
- Threonine363 is a major phosphorylation site of the kappa-opioid receptor (KOP). The pT363-KOP antibody detects phosphorylation in response to high-efficacy agonists. T363 phosphorylation is a key regulator of KOP desensitization, β-arrestin recruitment and internalization.
  
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Properties
- Host
  
  rabbit
  
  Antibody Type
  
  polyclonal
  
  Isotype
  
  IgG
  
  Reactivity
  
  human; mouse; rat
  
  Immunogen
  
  A synthetic phosphopeptide derived from human KOP around the phosphorylation site of Thr363
  
  Purification
  
  Antigen affinity chromatography
  
  Formulation
  
  Liquid
  
  Storage
  
  Short-term storage at 4°C and long-term storage at -20°C
  
  Antigen
  
  KOP, OPRK1, κ-Opioid Receptor (UniProt:P41145 (human), P33534 (mouse), P34975 (rat))
  
  * This product is intended for research use only.

Applications

Application	Dilutions
Western Blot (WB)	1:1000

Reference
- Chen C, Chiu YT, Wu W, Huang P, Mann A, Schulz S, Liu-Chen LY. Determination of sites of U50,488H-promoted phosphorylation of the mouse κ opioid receptor (KOPR): disconnect between KOPR phosphorylation and internalization. Biochem J. 2016 Feb 15;473(4):497-508. doi: 10.1042/BJ20141471. Epub 2015 Dec 3.
  Chiu YT, Chen C, Yu D, Schulz S, Liu-Chen LY. Agonist-Dependent and -Independent κ Opioid Receptor Phosphorylation: Distinct Phosphorylation Patterns and Different Cellular Outcomes. Mol Pharmacol. 2017 Nov;92(5):588-600. doi: 10.1124/mol.117.108555. Epub 2017 Sep 11. Erratum in: Mol Pharmacol. 2018 Aug;94(2):895. PubMed PMID: 28893975; PubMed Central PMCID: PMC5635518.