Name: Propidium Iodide (PI)
Price: 244.00 USD

- Overview
  - Propidium Iodide (PI) is a fluorescent nucleic acid dye that can be used for staining mammalian cells, bacteria, and yeast. It cannot penetrate the membrane of live cells but can pass through the membranes of dead cells and cells in the late stages of apoptosis to stain the nucleus. Therefore, PI is commonly used as a fluorescent probe for detecting apoptosis or necrosis. Once inside the cell, PI binds to DNA and RNA, increasing fluorescence intensity by 20 to 30 times. In flow cytometry, PI is often used in combination with other dyes such as Calcein-AM, Hoechst 33258, or Hoechst 33342 to distinguish between early and late apoptotic cells as well as dead cells. Additionally, PI is also frequently used as a counterstain in multicolor fluorescence staining.
    
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- Properties
  - Categories
    
    Nuclear Fluorescent Dyes
    
    Other Properties
    
    Validity: 12 months
    PI Excitation/Emission Wavelength(Unbound DNA): 493 / 636 nm
    PI Excitation/Emission Wavelength(Bound DNA): 535 / 617 nm
    
    Storage
    
    4°C, away from light
    
    Shipping
    
    Shipped with ice pack
    
    * For research use only
- Applications
  - Application Description
    
    Nuclear fluorescent dyes are a class of dyes that can bind to DNA in the cell nucleus and emit fluorescence. They play an important role in cell biology research. Commonly used nuclear fluorescent dyes include DAPI, Hoechst 33258, Hoechst 33342, PI (Propidium Iodide), and others. These nuclear fluorescent dyes provide a variety of options for observing and studying the cell nucleus due to their distinct fluorescence and staining properties.

Documents

Datasheet

Propidium Iodide (PI)

Propidium Iodide (PI)

Documents

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