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Overview
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DRAQ5 is a far-red fluorescent live-cell DNA dye and is an anthraquinone dye with high affinity for double-stranded DNA. It is a membrane-permeable dye that can label live cells or fixed/dead cells. In flow cytometry, this dye can be used to distinguish between nucleated and non-nucleated cells. Since DRAQ5 can bind to DNA in a stoichiometric ratio, it can also be used to report nuclear DNA content and is suitable for chromosome ploidy and cell cycle analysis. In fluorescence microscopy analysis, it can be used as a nuclear counterstain. DRAQ5 has many applications and is highly compatible with existing instrument platforms and widely used protocols. The main application areas are HCS (high-content screening), cell models, GFP (green fluorescent protein), flow cytometry, and fluorescence microscopy.
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