dsDNA Quantification Reagent (Green)

dsDNA Quantification Reagent (Green) is a product for fluorescent detection and quantification of dsDNA, and this method is highly sensitive. It is commonly used in molecular biology techniques such as the construction of cDNA libraries, purification of subcloned DNA fragments, and applications like DNA quantification, product amplification, and further detection of primers. The conventional method for detecting DNA content is to measure its absorbance at 260 nm. The main drawbacks of this method are that nucleotides, single-stranded nucleic acids, and proteins can significantly affect the signal, and it is also susceptible to interference from contaminants in the nucleic acid preparation process. It cannot distinguish between DNA and RNA, and it is not sensitive (A260 = 0.1 for a 5 μg/mL dsDNA solution). The dsDNA Quantification Reagent (Green) method is simple and convenient, and has become the standard for residual DNA detection in biological products.

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