mRNA-Fect In Vivo Kit - mRNA Transfection Reagent for Animal Models

Transfection reagents for mRNA delivery in both in vitro and in vivo models. mRNA-Fect In Vivo Kit is recommended for better efficacy in hard-to-transfect cell types, and mRNA-Fect In Vivo Kit contains 1 vial of mRNA-Fect and 1 vial of Trans-Booster.

The mRNA-Fect In Vivo Kit is a highly effective transfection reagent combination optimized for mRNA delivery in preclinical animal models. The kit components are based on synthetic amphiphilic polymers tailored primarily for mRNA delivery after extensive testing of polymer libraries. The components can undergo multivalent interactions with mRNA, encapsulating it into nanoparticles (~100 nm) appropriate for effective cellular uptake. This interaction occurs in aqueous buffer, obviating the need for organic solvents during complex preparation. The mRNA-Fect Kit components are non-integrating carriers, so that the genetic make-up of host cells is not altered after the treatment. These transfection reagents have been tested and found effective for plasmid DNA and siRNA delivery as well in certain cell types. As with all transfection reagents, formulations of mRNA-Fect In Vivo Kit with polynucleotides may need to be optimized for specific applications and transfection conditions.

Please contact us at for specific academic pricing.

Background

The mRNA-Fect reagent is a highly effective transfection reagent optimized for mRNA delivery to both attachment-dependent and suspension-growing cells. mRNA-Fect reagent is a synthetic amphiphilic polymer that is tailored primarily for mRNA delivery after extensive testing of amphiphilic polymer libraries. It is capable of undergoing multivalent interactions with polynucleotides and encapsulating coincubated mRNA into approximately 100 nm particles with a net positive charge. The complexation between the mRNA and the mRNA-Fect occurs in aqueous buffers, obviating the need for organic solvents during preparation. The mRNA-Fect is a non-integrating carrier, so that the genetic make-up of host cells is not altered after treatment with this reagent. The mRNA-Fect has been tested and found effective for plasmid DNA and siRNA delivery as well in certain cell types. As with all transfection reagents, formulations of mRNA-Fect with polynucleotides may need to be optimized for specific cell types and transfection conditions.