HIC Standard for Column Performance

HIC Standard for Column Performance

Catalog Number:
BRAK515979CEL
Mfr. No.:
CM92007
Price:
$413
  • Size:
    EA
    Quantity:
    Add to Cart:
      • Overview
        • The Hydrophobic Interaction chromatography (HIC) protein standard is designed for day to day use to check HIC column performance. The lyophilized protein mixture can be quickly reconstitution in water without any insoluble particles. The lyophilized product is also more stable for storage and can be shipped at ambient temperature.

          The kit consists of 4 different biomolecules with varying surface hydrophobicity. The Standard Mixture contains 3 proteins and 1 amino acid with MW range of 155 to 25,600 Da. The broad range of protein with different hydrophobic coverage allows one to test the performance of HIC column.

          A total of two micro-centrifuge tubes are in the package. The product is ready for use after reconstituting in 120 µL of deionized water.

          Key Features of the product:
          • Lyophilized solid. Ready to use for HPLC analysis after dissolving in 120 µL of deionized water.
          • Mixture of 3 high purity proteins and 1 amino acid of varying hydrophobic behavior with broad MW coverage: 155 to 25,600 Da

          Please contact us at for specific academic pricing.

      • Properties
        • * For Research Use Only. Not for Use in Diagnostic Procedures.

      • Applications
        • Application Description
          • Hydrophobic Interaction column performance testing
          • HIC HPLC Method development

          Application Note:
          The protein standard mixture can be used to assess HIC column performance. The HIC profile of the protein standards may differ from column to column after some use. The quality of the HIC profile will indicate whether the column is good. For example, split peaks may indicate column resin-bound hydrophobic molecules and that the column needs cleaning following column manufacturer recommendations.

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