pET28TEV-LwaCas13a Expression Plasmid

The pET28TEV-LwaCas13a plasmid is an expression plasmid for LwaCas13a (also known as LwaC2c2) protein, which is used to express and prepare LwaCas13a protein in Escherichia coli BL21 (DE3) strain. LwaCas13a nuclease is an RNA-mediated RNA endonuclease. When the PFS sequence exists in the target ss RNA, it can specifically recognize and cut the target RNA. In addition, Cas13a also has a trans-cleavage activity (i.e., bypass cleavage activity/collateral cleavage activity) that depends on the target ss RNA. In the presence of the target RNA, Cas13a can be activated for trans-cleavage activity against ssDNA, and the ssDNA in the system will be shredded. This activity is also used in the development of rapid detection kits for target nucleic acids. For example, Feng Zhang's team at the Broad Institute developed a next-generation molecular diagnostic system called "SHERLOCK" (PMID: 28408723) using the Cas13a protein. The plasmid contains a lactose operon inducible expression system, which can be induced to express the target protein by IPTG or lactose and its analogues.

Please contact us at for specific academic pricing.