pET28TEV-AsCas12a Expression Plasmid

The pET28TEV-AsCas12a plasmid is an expression plasmid of AsCas12a (also known as AsCpf1) protein, which is used to express and prepare AsCas12a protein in Escherichia coli BL21 (DE3) strain. AsCas12a is derived from Acidaminococcus sp.BV3L6 strain. AsCas12a is a DNA endonuclease mediated by crRNA. When the target double-stranded DNA has a PAM (TTTN) sequence, it specifically cuts the target double-stranded DNA, breaks the DNA double-strand and generates sticky ends. At the same time, when the AsCas12a protein combines with crRNA and target double-stranded DNA to form a ternary complex, it will be activated for trans-cleavage activity against ssDNA, and the ssDNA in the system will be shredded. Therefore, AsCas12a protein can be used not only for in vitro cleavage of target dsDNA, but also for the development of nucleic acid rapid detection kits, etc. The plasmid contains a lactose operon inducible expression system, which can be induced to express the target protein by IPTG or lactose and its analogues.

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