pET28TEV-BbCas12a Expression Plasmid

The pET28TEV-BbCas12a plasmid is an expression plasmid for BbCas12a (also known as BbCpf1) protein, which is used to express and prepare BbCas12a protein in Escherichia coli BL21 (DE3) strain. BbCas12a is derived from Bacteroidales bacterium KA00251 strain. BbCas12a is a DNA endonuclease mediated by crRNA. When the target double-stranded DNA has a PAM (TTN) sequence, it specifically cuts the target double-stranded DNA, breaks the DNA double-strand and generates sticky ends. At the same time, when BbCas12a protein combines with crRNA and target double-stranded DNA to form a ternary complex, it will be activated for trans-cleavage activity against ssDNA, and the ssDNA in the system will be shredded. Therefore, the BbCas12a protein can be used not only for in vitro cleavage of target dsDNA, but also for the development of nucleic acid rapid detection kits, etc. The plasmid contains a lactose operon inducible expression system, which can be induced to express the target protein by IPTG or lactose and its analogues.

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