pET28TEV-BoCas12a Expression Plasmid

The pET28TEV-BoCas12a plasmid is an expression plasmid for BoCas12a (also known as BoCpf1) protein, which is used to express and prepare BoCas12a protein in Escherichia coli BL21 (DE3) strain. BoCas12a is derived from Bacteroidetes oral taxon 274 strain. BoCas12a is a DNA endonuclease mediated by crRNA. When the target double-stranded DNA has a PAM (TTN) sequence, it specifically cuts the target double-stranded DNA, breaks the DNA double-strand and generates sticky ends. At the same time, when BoCas12a protein combines with crRNA and target double-stranded DNA to form a ternary complex, it will activate the trans-cleavage activity against ssDNA, and the ssDNA in the system will be shredded. Therefore, BoCas12a protein can be used not only for in vitro cleavage of target dsDNA, but also for the development of nucleic acid rapid detection kits, etc. The plasmid contains a lactose operon inducible expression system, which can be induced to express the target protein by IPTG or lactose and its analogues.

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