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Overview
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Ovalbumin (Ova) is a small protein sometimes used as a carrier protein for antibody production. Chicken egg albumin is the major protein constituent of egg whites and has a MW of 44.3 KDa if the carbohydrate and phosphate portions are accounted. This personalized Ova conjugation kit is designed to work with any peptide containing a reactive Cys group. Using the kit components, customers prepare the Ova-peptide conjugate by reacting their peptide (supplied by the customer) with activated Ova (supplied in the kit). A single purification step typically provides the resulting Ova-peptide at greater than 95% purity.
This kit provides materials to perform Ova labeling of one (CM52418x1) or three peptides (CM52418x3). Total amount of activated Ova included in a reaction: 2 mg.
• Requirement for Cys peptide:
Amount: 0.23–0.9 µmol (5–20 equivalents of peptide over Ova).
HPLC purified and lyophilized. Please make sure no reducing reagents, such as DTT, are present.
The Cys peptide should be stored at -80°C .
HPLC purity: >85% for N-terminal Cys peptide and >90% for C-terminal Cys peptide
• Key Features of this Ova-Peptide Conjugation Kit:
High quality maleimide-activated Ova for the conjugation: >95% purity
High maleimide groups per Ova for peptide loading: 10-20
A single purification affords over 95% of the Ova-peptide conjugates
Fast preparation: less than 1 hour hands-on time
All reagents included, from preparation to purificationPlease contact us at for specific academic pricing.
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Overview