PerKit™ F(ab')2 Preparation Kit

In the preparation of antibody-enzyme conjugates there are a few advantages of using antibody fragments rather than intact antibodies. For example, antibody fragments lacking an Fc region have less interference with various Fc binding proteins, can easily penetrate cell membranes due to their lower molecule weight, and experience a lower nonspecific binding to cell surfaces. F(ab')2 and Fab' fragments are also useful in immuno-histochemical studies.

This kit is designed for F(ab')2 preparation from mouse IgG1 antibody. The procedure we use involves cleaving the IgG molecules below the disulfide groups in the hinge region using Ficin to create a bivalent fragment and degraded smaller peptide fragments, and then purify to obtain F(ab')2.

F(ab')2 can be further reduced to produce Fab' fragment. This kit provides materials to fragment 0.25-3 mg of mouse IgG1 with the option to choose from one (CM51408x1) or three (CM51408x3) reactions.

• Key Features of this PerKit™
Easy preparation: 1 day preparation and <2 h hands-on time
All reagents and supplies included for preparation and purification
40~60% recovery and 80-95% of pure F(ab')2 by SEC (size-exclusion chromatography)

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