pAAVone-AAVrh.10-CMV-EGFP-2.2kb

pAAVone-AAVrh.10-CMV-EGFP-2.2kb is a pAAVone plasmid used to produce 2.2kb of AAVrh.10-CMV-EGFP using novel AAVone production system. It integrates all the AAV production necessary components, including Ad helper genes (E2A, E4orf6 and VA RNA), AAV helper genes (Rep and AAVrh.10), and a single-strand AAV-CMV-EGFP genome. AAVrh.10-CMV-EGFP viruses can be simply and efficiently produced by transfection of this single-plasmid into host cells, such us HEK 293 and HEK 293T. AAVrh.10 is a serotype of AAV that was originally isolated from rhesus monkeys. AAVrh.10 can efficiently target the CNS, liver, lungs, heart, and muscle tissues, among others. Its ability to cross the blood-brain barrier (BBB) makes it particularly valuable for delivering therapeutic genes to the brain.

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Background

Our data showed that AAVrh.10 delivers high levels of RNA and DNA across various tissues in C57BL/6 mice following intravenous (IV) injection.pAAVone plasmids are used to produce AAVs using novel AAVone® signle-plasmid AAV production system. They integrates all the AAV production necessary components, including Ad helper genes (E2A, E4orf6 and VA RNA), AAV helper genes (Rep and Cap), and AAV genome. To produce AAVs, users only need select the pAAVone plasmid carrying the corresponding capsid, clone your transgenes into it and transfect them into host cells. Pre-made pAAVone plasmids are available in the following serotypes: AAV1, AAV2, AAV2-Retro, AAV3B, AAV4, AAV6, AAV7, AAV8, AAV9, AAV11, AAV12, AAV13, AAV-DJ, AAVrh.10, AAVrh.74, and AAV-PHP.eB.