pAAVone-AAV8-CMV-EGFP-2.8kb

pAAVone-AAV8-CMV-EGFP-2.8kb is a pAAVone plasmid used to produce AAV8 Reference (Full) and AAV8 Reference (Byproduct-Empty) using novel AAVone production system. It integrates all the AAV production necessary components, including Ad helper genes (E2A, E4orf6 and VA RNA), AAV helper genes (Rep and AAV8), and a single-strand AAV-CMV-EGFP genome. AAV8-CMV-EGFP viruses can be simply and efficiently produced by transfection of this single-plasmid into host cells, such us HEK 293 and HEK 293T. AAV8 is a naturally occurring adeno-associated virus serotype that has been widely used in gene therapy due to its strong tropism for the liver, heart, and muscle tissues. It is known for its superior ability to transduce hepatocytes, making it a leading choice for liver-targeted gene therapies. AAV8 is also effective in delivering genes to other tissues such as cardiac and skeletal muscle.

Please contact us at for specific academic pricing.

Background

Our data showed that AAV8 delivers high levels of RNA and DNA across various tissues in C57BL/6 mice following intravenous (IV) injection.pAAVone plasmids are used to produce AAVs using novel AAVone® signle-plasmid AAV production system. They integrates all the AAV production necessary components, including Ad helper genes (E2A, E4orf6 and VA RNA), AAV helper genes (Rep and Cap), and AAV genome. To produce AAVs, users only need select the pAAVone plasmid carrying the corresponding capsid, clone your transgenes into it and transfect them into host cells. Pre-made pAAVone plasmids are available in the following serotypes: AAV1, AAV2, AAV2-Retro, AAV3B, AAV4, AAV6, AAV7, AAV8, AAV9, AAV11, AAV12, AAV13, AAV-DJ, AAVrh.10, AAVrh.74, and AAV-PHP.eB.