pAAVone-AAV1-CMV-EGFP-2.2kb

pAAVone-AAV1-CMV-EGFP-2.2kb is a pAAVone plasmid used to produce AAV1-CMV-EGFP using novel AAVone production system. It integrates all the AAV production necessary components, including Ad helper genes (E2A, E4orf6 and VA RNA), AAV helper genes (Rep and AAV1), and a 2.2kb single-strand AAV-CMV-EGFP genome. AAV1-CMV-EGFP viruses can be simply and efficiently produced by transfection of this single-into host cells, such us HEK 293 and HEK 293T. AAV1 is a serotype of adeno-associated virus that was originally isolated from rhesus monkeys. AAV1 can efficiently target the CNS, liver, lungs, heart, and muscle tissues, among others.

Please contact us at for specific academic pricing.

Background

Our data revealed that AAV1 shows relatively high RNA expression in the heart and kidney, but relatively high DNA levels in the liver of C57B6 mice following intravenous (IV) injection.pAAVone plasmids are used to produce AAVs using novel AAVone® signle-plasmid AAV production system. They integrates all the AAV production necessary components, including Ad helper genes (E2A, E4orf6 and VA RNA), AAV helper genes (Rep and Cap), and AAV genome. To produce AAVs, users only need select the pAAVone plasmid carrying the corresponding capsid, clone your transgenes into it and transfect them into host cells. Pre-made pAAVone plasmids are available in the following serotypes: AAV1, AAV2, AAV2-Retro, AAV3B, AAV4, AAV6, AAV7, AAV8, AAV9, AAV11, AAV12, AAV13, AAV-DJ, AAVrh.10, AAVrh.74, and AAV-PHP.eB.