pAAVdual-hSyn-iCre

pAAVdual-hSyn-iCre is used to produce AAV-hSyn-iCre virus using novel AAVdual production system. In this plasmid, the regular single strand AAV genome with a hSyn promoter and an iCre reporter is cloned into our novel Ad helper plasmid, mini-pHelper. AAV-hSyn-iCre viruses can be generated by co-transfection of this plasmid with regular AAV helper (pRCap) plasmids, carrying AAV2 rep gene and different cap genes, without adding additional Ad helper plasmid to supply E2A, E4orf6 and VA RNA functions.
The hSyn promoter, also known as the human Synapsin 1 promoter, is a regulatory sequence used to drive gene expression specifically in neurons. Synapsin 1 is a neuron-specific phosphoprotein that plays a crucial role in the regulation of neurotransmitter release at synapses. The hSyn promoter is a neuron-specific regulatory element that drives strong and selective gene expression in neurons. It is widely used in neuroscience research, gene therapy, the development of transgenic models, and optogenetic studies. Its specificity to neurons makes it invaluable for targeting gene expression in the nervous system, facilitating research into neuronal function, connectivity, and neurological disorders.
iCre is a codon-optimized variant of Cre recombinase, designed to enhance its expression and efficiency in mammalian cells. Like the standard Cre recombinase, iCre recognizes loxP sites and catalyzes site-specific recombination between them, which can result in the deletion, inversion, or translocation of the DNA sequence flanked by these sites. AAV-iCre offers an improved and more efficient approach to conditional gene manipulation, providing researchers with a powerful tool for studying gene function, lineage tracing, and gene editing.

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