pAAVdual-GFAP-Cre

pAAVdual-GFAP-Cre is used to produce AAV-GFAP-Cre virus using novel AAVdual production system. In this plasmid, the regular single strand AAV genome with a GFAP promoter and an Cre reporter is cloned into our novel Ad helper plasmid, mini-pHelper. AAV-GFAP-Cre viruses can be generated by co-transfection of this plasmid with regular AAV helper (pRCap) plasmids, carrying AAV2 rep gene and different cap genes, without adding additional Ad helper plasmid to supply E2A, E4orf6 and VA RNA functions.
The GFAP promoter refers to the regulatory sequence that controls the expression of the Glial Fibrillary Acidic Protein (GFAP) gene. GFAP is a key intermediate filament protein that is predominantly expressed in astrocytes, which are star-shaped glial cells in the central nervous system (CNS). The GFAP promoter is a glial-specific regulatory element that drives gene expression primarily in astrocytes within the central nervous system. It is a powerful tool in neuroscience research, gene therapy, and the development of transgenic models for studying astrocyte function, CNS development, and neurodegenerative diseases. Its specificity to astrocytes makes it invaluable for targeting gene expression in studies focused on glial cell biology, CNS health, and pathology.
Cre recombinase is a site-specific DNA recombinase enzyme derived from the bacteriophage P1. Cre recombinase recognizes specific DNA sequences known as loxP sites and catalyzes the recombination between them. This recombination event can lead to the deletion, inversion, or translocation of the DNA segment flanked by loxP sites, depending on the orientation of the sites. AAV-Cre is a powerful tool for genetic research, enabling targeted manipulation of genes in specific tissues or at specific times. Its ability to drive site-specific recombination makes it an essential component of many gene editing, knockout, and lineage tracing studies.

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