pAAVdual-CaMKIIα(0.36)-Cre

pAAVdual-CaMKIIα(0.36)-Cre is used to produce AAV-CaMKIIα(0.36)-Cre virus using novel AAVdual production system. In this plasmid, the regular single strand AAV genome with a CaMKIIα(0.36) promoter and an Cre reporter is cloned into our novel Ad helper plasmid, mini-pHelper. AAV-CaMKIIα(0.36)-Cre viruses can be generated by co-transfection of this plasmid with regular AAV helper (pRCap) plasmids, carrying AAV2 rep gene and different cap genes, without adding additional Ad helper plasmid to supply E2A, E4orf6 and VA RNA functions.
The 0.36kb CaMKIIα promoter refers to a shortened version of the promoter region for the Calcium/Calmodulin-Dependent Protein Kinase II Alpha (CaMKIIα) gene. CaMKIIα is a crucial enzyme in the brain, particularly in neurons, where it plays a significant role in synaptic plasticity, learning, and memory. This 0.36kb version of the promoter is specifically designed to drive gene expression in neurons, particularly in excitatory neurons of the forebrain, such as those in the hippocampus and cortex.
Cre recombinase is a site-specific DNA recombinase enzyme derived from the bacteriophage P1. Cre recombinase recognizes specific DNA sequences known as loxP sites and catalyzes the recombination between them. This recombination event can lead to the deletion, inversion, or translocation of the DNA segment flanked by loxP sites, depending on the orientation of the sites. AAV-Cre is a powerful tool for genetic research, enabling targeted manipulation of genes in specific tissues or at specific times. Its ability to drive site-specific recombination makes it an essential component of many gene editing, knockout, and lineage tracing studies.

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