pAAVdual-CaMKIIα-Cluc

pAAVdual-CaMKIIα-Cluc is used to produce AAV-CaMKIIα-Cluc virus using novel AAVdual production system. In this plasmid, the regular single strand AAV genome with a CaMKIIα promoter and an Cluc reporter is cloned into our novel Ad helper plasmid, mini-pHelper. AAV-CaMKIIα-Cluc viruses can be generated by co-transfection of this plasmid with regular AAV helper (pRCap) plasmids, carrying AAV2 rep gene and different cap genes, without adding additional Ad helper plasmid to supply E2A, E4orf6 and VA RNA functions.
The CaMKIIα promoter refers to the regulatory sequence that controls the expression of the Calcium/Calmodulin-Dependent Protein Kinase II Alpha (CaMKIIα) gene. CaMKIIα is an essential enzyme predominantly expressed in the brain, especially in excitatory neurons of the forebrain, such as those found in the hippocampus and cortex. The CaMKIIα promoter is a neuron-specific regulatory element that drives gene expression in excitatory neurons of the forebrain, particularly in regions like the hippocampus and cortex. It is a critical tool in neuroscience research, transgenic model development, optogenetics, and potentially gene therapy. Its specificity to neurons involved in synaptic plasticity, learning, and memory makes it invaluable for studying the molecular and cellular mechanisms underlying these key brain functions.
Cypridina luciferase (Cluc), a bioluminescent reporter protein derived from the marine ostracod Cypridina noctiluca. Cluc emits light in the blue range when it catalyzes the oxidation of its substrate, Cypridina luciferin. This property makes Cluc an excellent tool for various biological research applications, particularly in non-invasive imaging and real-time monitoring of biological processes. AAV-Cluc is a powerful tool for researchers looking to perform sensitive, non-invasive imaging and real-time monitoring of gene expression and biological processes. Its high sensitivity and low background make it particularly useful for in vivo studies and applications where continuous, non-destructive observation is required.

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