pAAVdual-CAG-Cluc

pAAVdual-CAG-Cluc is used to produce AAV-CAG-Cluc virus using novel AAVdual production system. In this plasmid, the regular single strand AAV genome with a CAG promoter and an Cluc reporter is cloned into our novel Ad helper plasmid, mini-pHelper. AAV-CAG-Cluc viruses can be generated by co-transfection of this plasmid with regular AAV helper (pRCap) plasmids, carrying AAV2 rep gene and different cap genes, without adding additional Ad helper plasmid to supply E2A, E4orf6 and VA RNA functions.
The CAG promoter is a synthetic hybrid promoter widely used in molecular biology to drive high levels of gene expression in mammalian cells. It is composed of three key elements:CMV Immediate-Early Enhancer: Derived from the cytomegalovirus (CMV), this enhancer sequence provides strong and ubiquitous transcriptional activation in a variety of cell types. Chick Beta-Actin Promoter: The core promoter region is derived from the chicken beta-actin gene, which is known for its high expression levels in many cell types, particularly in the context of gene expression in vertebrates. Rabbit Beta-Globin Intron: An intronic sequence from the rabbit beta-globin gene is included to enhance transcriptional efficiency and mRNA stability, further boosting expression levels. It is particularly valued for its strength and broad activity across various cell types, making it a popular choice for applications such as gene therapy, transgenic animal models, and research studies that require robust and consistent gene expression.
Cypridina luciferase (Cluc), a bioluminescent reporter protein derived from the marine ostracod Cypridina noctiluca. Cluc emits light in the blue range when it catalyzes the oxidation of its substrate, Cypridina luciferin. This property makes Cluc an excellent tool for various biological research applications, particularly in non-invasive imaging and real-time monitoring of biological processes. AAV-Cluc is a powerful tool for researchers looking to perform sensitive, non-invasive imaging and real-time monitoring of gene expression and biological processes. Its high sensitivity and low background make it particularly useful for in vivo studies and applications where continuous, non-destructive observation is required.

Please contact us at for specific academic pricing.