pAAVdual-CAG-iCre

pAAVdual-CAG-iCre is used to produce AAV-CAG-iCre virus using novel AAVdual production system. In this plasmid, the regular single strand AAV genome with a CAG promoter and an iCre reporter is cloned into our novel Ad helper plasmid, mini-pHelper. AAV-CAG-iCre viruses can be generated by co-transfection of this plasmid with regular AAV helper (pRCap) plasmids, carrying AAV2 rep gene and different cap genes, without adding additional Ad helper plasmid to supply E2A, E4orf6 and VA RNA functions.
The CAG promoter is a synthetic hybrid promoter widely used in molecular biology to drive high levels of gene expression in mammalian cells. It is composed of three key elements:CMV Immediate-Early Enhancer: Derived from the cytomegalovirus (CMV), this enhancer sequence provides strong and ubiquitous transcriptional activation in a variety of cell types. Chick Beta-Actin Promoter: The core promoter region is derived from the chicken beta-actin gene, which is known for its high expression levels in many cell types, particularly in the context of gene expression in vertebrates. Rabbit Beta-Globin Intron: An intronic sequence from the rabbit beta-globin gene is included to enhance transcriptional efficiency and mRNA stability, further boosting expression levels. It is particularly valued for its strength and broad activity across various cell types, making it a popular choice for applications such as gene therapy, transgenic animal models, and research studies that require robust and consistent gene expression.
iCre is a codon-optimized variant of Cre recombinase, designed to enhance its expression and efficiency in mammalian cells. Like the standard Cre recombinase, iCre recognizes loxP sites and catalyzes site-specific recombination between them, which can result in the deletion, inversion, or translocation of the DNA sequence flanked by these sites. AAV-iCre offers an improved and more efficient approach to conditional gene manipulation, providing researchers with a powerful tool for studying gene function, lineage tracing, and gene editing.

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