OneScript® Plus Reverse Transcriptase

- Overview
  - OneScript® Plus Reverse Transcriptase is a Moloney-Murine Leukemia Virus Reverse Transcriptase with genetic modifications to abolish RNase H activity to achieve thermal stability. This special mutant enzyme offers higher cDNA yields, longer cDNA up to 12 kb, and is able to perform under high temperatures (50°C - 55°C), facilitating the elimination of secondary structures associated with GC-rich RNA templates.
    OneScript® Plus is formulated with the RNaseOFF Ribonuclease Inhibitor (Cat. No. G138) offering improved resistance to oxidation compared to the high oxidation-sensitive human RNase inhibitors. RNaseOFF is stable even under very low concentrations of DTT (< 1 mM), making it the best choice for ultimate RNA protection.
    
    Product Features:
    High thermostability at 50-55°C/122-131°F
    Synthesize 15 kb RNA Templates
    10-15 minutes reaction time
    Earn 2X the rewards points
    OneScript® Plus cDNA Synthesis Kit (Cat. No. G236) also available
    Pair with BlasTaq™ 2X PCR MasterMix (Cat. No. G895) for Two-Step RT-PCR workflows
    
    Please contact us at for specific academic pricing.
- Properties
  - Categories
    
    Reverse Transcription and RT-PCR
    
    Storage
    
    Store at -20°C.
    
    * For research use only. Not for diagnostic or therapeutic use.
- Applications
  - Application Description
    
    Synthesizing cDNA from a ssRNA
    DNA primer extension
    Sequencing dsDNA
    Constructing cDNA library
    Constructing libraries for serial analysis for gene expression (SAGE)
    Synthesizing cDNA in rapid amplification of cDNA ends (3’ & 5’ RACE)
    Producing template for use in RT-PCR or real-time RT-PCR
    Labelling 3'-end of duplex DNA via end-filling reactions
    Generating probes for hybridization
- Reference
  - Menon, V et al. "The contribution of visceral fat to improved insulin signaling in Ames dwarf mice" Aging Cell 13 (3):497-506 (2014). DOI: 10.1111/acel.12201. PubMed: 24690289. Application: Reverse Transcription.
    Lee, G et al. "Potential monoclonal antibody therapy for the treatment of ovarian cancer" Ovarian cancer—basic science perspective :385-406 (2012). DOI: 10.5772/1268 . Application: Reverse Transcription.
    Correnti, JM et al. "Adiponectin fine-tuning of liver regeneration dynamics revealed through cellular networkmodeling" J Physiol. : (2014). PubMed: 25384784.
    Correnti, JM et al. "Adiponectin fine-tuning of liver regeneration dynamics revealed through cellular network modelling" J Physiol 593(2):365-383 (2015). DOI: 10.1113/jphysiol.2014.284109. PubMed: 25630259. Application: Reverse Transcription.
    Guo, C., Wang, X., Zhang, H., Zhi, L., Lv, T., Li, M., … Zhu, W. "Structure-based rational design of a novel chimeric PD1-NKG2D receptor for natural killer cells" Molecular Immunology 114:108–113 (2019). DOI: 10.1016/j.molimm.2019.07.009.
    Wu, F., Niu, Z., Zhou, B., Li, P., & Qian, F. "PSMB1 Negatively Regulates the Innate Antiviral Immunity by Facilitating Degradation of IKK-ε" Viruses 11(2):99 (2019). DOI: 10.3390/v11020099.
    Zhao, G., Zhang, T., Sun, H. J., & Liu, J. "Copper nanoparticles induce zebrafish intestinal defects via endoplasmic reticulum and oxidative stress" Metallomics. : (2019).

Datasheet

MSDS

OneScript® Plus Reverse Transcriptase

OneScript® Plus Reverse Transcriptase

We Also Recommend

All-In-One 5X RT MasterMix

BlasTaq™ 2X qPCR MasterMix

BlasTaq™ DNA Polymerase