All-In-One 5X RT MasterMix

All-In-One 5X RT MasterMix

Catalog Number:
PER1279050APP
Mfr. No.:
APPL_G592
Price:
$436
  • Size:
    100 rxn
    Quantity:
    Add to Cart:
      • Overview
        • Enjoy accurate gDNA-free cDNA synthesis!
          All-In-One 5X RT MasterMix is a convenient and ready-to-use formulation for first-strand cDNA synthesis, including genomic DNA (gDNA) removal. Genomic DNA contamination is a common problem for accurate RNA detection and this MasterMix solves that problem without affecting reverse transcription and first-strand cDNA synthesis. cDNA synthesis will be simple, reliable, and reproducible. The optimized 5X RT MasterMix contains the proprietary OneScript® Hot Reverse Transcriptase, RNaseOFF Ribonuclease Inhibitor, temperature-sensitive DNase, dNTPs, and a finely-balanced ratio of Oligo (dT)s and Random Primers. The high-quality cDNA synthesized from this kit can be used for a wide range of downstream applications.

          Product Features:
          Easy one-step setup reduces pipetting and sample handling
          High cDNA yields from difficult samples with OneScript® Hot Reverse Transcriptase (Cat. No. G593)
          Inhibits ribonuclease contaminants with RNaseOFF Ribonuclease Inhibitor (Cat. No. G591)
          Removes contaminating gDNA with temperature-sensitive DNase
          Also includes dNTPs, Oligo (dT)s, and random primers
          Earn 2X the rewards points

          Please contact us at for specific academic pricing.

      • Properties
        • Categories
          Reverse Transcription and RT-PCR
          Concentration
          5X
          Storage
          Store at -20°C.

          * For research use only. Not for diagnostic or therapeutic use.

      • Applications
        • Application Description
          Generation of templates for use in RT-PCR and qRT-PCR
          cDNA synthesis from ssRNA
          cDNA library construction
          Generation of probes for hybridization
          DNA primer extension
      • Reference
        • Lee, G et al. "Potential monoclonal antibody therapy for the treatment of ovarian cancer" Ovarian cancer—basic science perspective :385-406 (2012). DOI: 10.5772/1268 . Application: Reverse Transcription.
          Wu, F., Niu, Z., Zhou, B., Li, P., & Qian, F. "PSMB1 Negatively Regulates the Innate Antiviral Immunity by Facilitating Degradation of IKK-ε" Viruses 11(2):99 (2019). DOI: 10.3390/v11020099.
          Zhao, G., Zhang, T., Sun, H. J., & Liu, J. "Copper nanoparticles induce zebrafish intestinal defects via endoplasmic reticulum and oxidative stress" Metallomics. : (2019).

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