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Overview
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Enjoy accurate gDNA-free cDNA synthesis!
All-In-One 5X RT MasterMix is a convenient and ready-to-use formulation for first-strand cDNA synthesis, including genomic DNA (gDNA) removal. Genomic DNA contamination is a common problem for accurate RNA detection and this MasterMix solves that problem without affecting reverse transcription and first-strand cDNA synthesis. cDNA synthesis will be simple, reliable, and reproducible. The optimized 5X RT MasterMix contains the proprietary OneScript® Hot Reverse Transcriptase, RNaseOFF Ribonuclease Inhibitor, temperature-sensitive DNase, dNTPs, and a finely-balanced ratio of Oligo (dT)s and Random Primers. The high-quality cDNA synthesized from this kit can be used for a wide range of downstream applications.
Product Features:
Easy one-step setup reduces pipetting and sample handling
High cDNA yields from difficult samples with OneScript® Hot Reverse Transcriptase (Cat. No. G593)
Inhibits ribonuclease contaminants with RNaseOFF Ribonuclease Inhibitor (Cat. No. G591)
Removes contaminating gDNA with temperature-sensitive DNase
Also includes dNTPs, Oligo (dT)s, and random primers
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Overview