BlasTaq™ 2X qPCR MasterMix

BlasTaq™ 2X qPCR MasterMix

Catalog Number:
PER1279052APP
Mfr. No.:
APPL_G891
Price:
$333
  • Size:
    500 rxn (4 x 1.25 ml)
    Quantity:
    Add to Cart:
      • Overview
        • BlasTaq™ 2X qPCR MasterMix provides a convenient, reliable and robust setup for performing quantitative real-time analysis of DNA samples. This ready-to-use qPCR MasterMix contains the strategically-engineered, next generation Taq Polymerase, BlasTaq™ DNA Polymerase, providing for rapid extension rates and robust performance. With specialized reaction conditions, this polymerase provides increased processivity, yields, and sensitivity, while shortening reaction times by up to 70%, compared to wild-type Taq DNA polymerase.
          BlasTaq™ has 5’-3’ polymerase and 5’-3’ exonuclease activities, lacks 3’-5’ exonuclease activity, and produces 3’-dA-tailed amplicons. qPCR products made with BlasTaq™ can be used with TA cloning vectors.

          Product Features:
          Enable streamlined protocol in a simple reaction set-up
          Allow accurate quantification of a variety of gene targets
          Reduce pipetting steps to minimize the risk of contamination
          Compatible with most real-time PCR instruments
          Contains dye comparable to SYBR Green™ and EvaGreen™ (Probe-based qPCR MasterMix also available)
          Earn 2X the rewards points

          Please contact us at for specific academic pricing.

      • Properties
        • Categories
          PCR Polymerases
          Concentration
          2X
          Storage
          Store at -20 °C.

          * For research use only. Not for diagnostic or therapeutic use.

      • Reference
        • Chung, G et al. "CHL-1 provides an essential function affecting cell proliferation and chromosome stability in Caenorhabditis elegans" DNA Repair (Amst.) 10 (11):1174-1182 (2011). DOI: 10.1016/j.dnarep.2011.09.011. PubMed: 21968058. Application: PCR.
          Liang, Y., Song, X., Li, Y., Su, P., Han, D., Ma, T., … Yang, Q. "circKDM4C suppresses tumor progression and attenuates doxorubicin resistance by regulating miR-548p/PBLD axis in breast cancer" Oncogene 38(42):6850–6866 (2019). DOI: 10.1038/s41388-019-0926-z.
          Verma, M., Asakura, Y., & Asakura, A. "Inhibition of microRNA‐92a increases blood vessels and satellite cells in skeletal muscle but does not improve duchenne muscular dystrophy–related phenotype in mdx mice" Muscle & Nerve 59(5):594–602 (2019). DOI: 10.1002/mus.26433.
          Wu., Chien-Wei., . "Downregulation of MiR-144 by Triptolide Enhanced p85α−PTEN Complex Formation Causing S Phase Arrest of Human Nasopharyngeal Carcinoma Cells" European Journal of Pharmacology 855:137-148 (2019). DOI: 10.1016/j.ejphar.2019.04.052..
          Wu, M.-J., Chen, Y.-S., Kim, M. R., Chang, C.-C., Gampala, S., Zhang, Y., … Chang, C.-J. "Epithelial-Mesenchymal Transition Directs Stem Cell Polarity via Regulation of Mitofusin" Cell Metabolism 29(4):993–1002.e6 (2019). DOI: 10.1016/j.cmet.2018.11.004.
          Xiang, X., Zhou, Y., Sun, H., Tan, S., Lu, Z., Huang, L., & Wang, W. "Ivabradine abrogates TNF-α-induced degradation of articular cartilage matrix" International Immunopharmacology 66:347–353 (2019). DOI: 10.1016/j.intimp.2018.11.035.
          Zhou, Y., Lei, J., Xie, Q., Wu, L., Jin, S., Guo, B., ... & Zhang, J. "Fibrinogen-like protein 2 controls sepsis catabasis by interacting with resolvin Dp5" Science Advances 5(11):eaax0629 (2019).
          Zhu, R., Xue, X., Shen, M., Tsai, Y., Keng, P. C., Chen, Y., … Chen, Y. "NFκB and TNFα as individual key molecules associated with the cisplatin-resistance and radioresistance of lung cancer" Experimental Cell Research 374(1):181–188 (2019). DOI: 10.1016/j.yexcr.2018.11.022.
          Yang, S., Tian, M., & Johnson, A. N. (2020). SARS-CoV-2 protein ORF3a is pathogenic inDrosophilaand causes phenotypes associated with COVID-19 post-viral syndrome. SARS-CoV-2 Protein ORF3a Is Pathogenic in Drosophila and Causes Phenotypes Associated with COVID-19 Post-Viral Syndrome. Published. https://doi.org/10.1101/2020.12.20.423533
          Yang, Y. W., Meng, X., Meng, Y. Y., Tang, H. K., Cheng, M. H., Zhang, Z. Q., Xu, W. Q., & Long, W. (2021). ceRNA regulatory network of FIH inhibitor as a radioprotector for gastrointestinal toxicity by activating the HIF-1 pathway. Molecular Therapy - Nucleic Acids, 25, 173–185. https://doi.org/10.1016/j.omtn.2021.05.008
          Luo, N., Cheng, W., Zhou, Y., Gu, B., Zhao, Z., & Zhao, Y. (2021). Screening Candidate Genes Regulating Placental Development from Trophoblast Transcriptome at Early Pregnancy in Dazu Black Goats (Capra hircus). Animals, 11(7), 2132. https://doi.org/10.3390/ani11072132
          Elizabeth L Mahon, Lisanne de Vries, Soo-Kyeong Jang, Sandeep Middar, Hoon Kim, Faride Unda, John Ralph, Shawn D Mansfield, Exogenous chalcone synthase expression in developing poplar xylem incorporates naringenin into lignins, Plant Physiology, Volume 188, Issue 2, February 2022, Pages 984–996, https://doi.org/10.1093/plphys/kiab499
          Sutthinon, P., Rungwattana, K., Suwanphakdee, C., Himaman, W., & Lueangjaroenkit, P. (2021). Endophytic Fungi from Root of Three Lady’s Slipper Orchids (Paphiopedilum spp.) in Southern Thailand. Chiang Mai Journal of Science, 48(3), 853–866.
          Li, M. X., Wu, G., & Yang, Z. L. (2021). Four New Species of Hemileccinum (Xerocomoideae, Boletaceae) from Southwestern China. Journal of Fungi, 7(10), 823.

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