OneScript® Hot Reverse Transcriptase

OneScript® Hot Reverse Transcriptase

Catalog Number:
PER1279048APP
Mfr. No.:
APPL_G593
Price:
$424
  • Size:
    100 rxn
    Quantity:
    Add to Cart:
      • Overview
        • OneScript® Hot Reverse Transcriptase is a mutational derivative of Moloney-Murine Leukemia Virus Reverse Transcriptase, that can reverse transcribe low abundance or degraded RNA, and has significantly better resistance to contaminating inhibitors such as reagents used during RNA extraction and contaminants from biological samples. High processivity and sensitivity allow for rapid cDNA synthesis of full-length cDNA fragments in a fraction of the time of leading competitors.
          The reverse transcriptase engineered offers superior cDNA synthesis performance with even the most challenging RNA samples due to its incredible thermostability at 60-72°C. This is the enzyme of choice for daily or demanding RNA reverse transcription. OneScript® Hot is formulated with the RNaseOFF Ribonuclease Inhibitor offering improved resistance to oxidation compared to the high oxidation-sensitive human RNase inhibitors. RNaseOFF is stable even under very low concentrations of DTT (< 1 mM), making it the best choice for ultimate RNA protection.

          Product Features:
          Thermostable from 60-72°C/140-161°F
          Transcribes degraded samples and resists inhibitors/contaminants
          Provides high cDNA yields from difficult or ultra-low RNA samples
          Earn 2X the rewards points

          Please contact us at for specific academic pricing.

      • Properties
        • Categories
          Reverse Transcription and RT-PCR
          Storage
          Store at -20°C.

          * For research use only. Not for diagnostic or therapeutic use.

      • Reference
        • Lee, G et al. "Potential monoclonal antibody therapy for the treatment of ovarian cancer" Ovarian cancer—basic science perspective :385-406 (2012). DOI: 10.5772/1268 . Application: Reverse Transcription.
          Wu, F., Niu, Z., Zhou, B., Li, P., & Qian, F. "PSMB1 Negatively Regulates the Innate Antiviral Immunity by Facilitating Degradation of IKK-ε" Viruses 11(2):99 (2019). DOI: 10.3390/v11020099.
          Zhao, G., Zhang, T., Sun, H. J., & Liu, J. "Copper nanoparticles induce zebrafish intestinal defects via endoplasmic reticulum and oxidative stress" Metallomics. : (2019).

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