Endo-Beta-Galactosidase

- Overview
  - Endo-β-galactosidase cleaves internal β(1-4) galactose linkages in unbranched, repeating poly-N-acetyllactosamine structures. Sulfated structures such as keratan sulfate are also cleaved. Branching and/or fucosylation of the substrate may decrease or eliminate cleavage.
    
    Endo-β-Galactosidase is useful for identifying and removing poly-N-acetyllactosamine structures on many biologically important glycoconjugates.
    
    Specificity: Cleaves internal β(1-4) galactose linkages in unbranched, repeating poly-N-acetyllactosamine [GlcNAc-β(1-3)Gal-β(1-4)]n structures are the preferred substrate. Sulfated structures such as keratan sulfate are also cleaved. Branching and/or fucosylation of the substrate may decrease or eliminate cleavage. Sulfation of C-6 on galactose will block cleavage. Oligosaccharidesof the neo-lacto group are cleaved at greatly educed rates depending on the deviation from the preferred substrate. For example, Gal-β(1-3)GlcNAc-β(1-3)Gal-β(1-4)Glc is cleaved at 5×10-5 the rate of keratan sulfate (see ref.4). Specificity is similar to the Escherichia freundii enzyme except that it is limited to cleaving N-acetyllactosamine extensions on tetraantennary structures of erythropoietin (see ref 5).
    
    Contents:
    60 µL aliquot of enzyme (0.9 U) in 20 mM tris-HCl, pH 7.5
    1 vial reaction buffer- 250mM Sodium phosphate, pH 5.8
    
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    More Details
    
    Glycosidases
- Properties
  - Source
    
    Recombinant from Bacteroides fragilis in E. Coli
    
    Purification
    
    Endo-β-Galactosidase is tested for contaminating protease as follows: 10 µg of denatured BSA is incubated for 24 hours at 37°C with 2 µL of enzyme. SDS-PAGE analysis of the treated BSA shows no evidence of degradation.
    
    Note
    
    Stable at least 24 months when stored properly. Several days exposure to ambient temperature will not reduce activity. Active for at least 5 days under reaction conditions.
    
    Activity
    
    >14 U/mL
    
    More Information
    
    Database Link
    
    EC 3.2.1.103
    
    * For research use only
- Applications
  - Application
    
    Glycan Release

Documents

Specsheet

CofA

MSDS

Endo-Beta-Galactosidase

More Details

More Information

Documents

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