Nystatin ReadyMade™ Solution

- Overview
  - Nystatin ReadyMadeTM solution is provided as a sterile-filtered solution of Nystatin formulated in 0.2N Hydrochloric Acid (HCl) at a concentration of 20,000-40,000 IU/ml. It has been filter-sterilized using a 0.2 μm filter.
    Nystatin is a fungicidal and fungistatic polyene antifungal and growth promoter. It functions by accumulating cholesterol and thereby sequesters lipid from cell membranes. It can be used in quantum dot research, since cholesterol depletion could block several lipid raft-dependent endocytic pathways. It was discovered in 1950 by Rachel Fuller Brown and Elizabeth Lee Hazen and was the first polyene macrolide antifungal, named after New York State. Commercially,it was produced at industrial scale by Squibb & Sons in 1954.
    
    Please contact us at for specific academic pricing.
    
    Background
    
    Nystatin binds ergosterol in the fungal cell membrane forming pores and increasing permeability. It is a channel-forming ionophore, meaning it forms a membrane-spanning pore in teh fungal plasma membrane. The altered permeability is toxic to the fungi leading to growth inhibition or death. It has a similar mechanism to Amphotericin B. It is not active against bacteria since they do not contain sterols in their cell membrane.
- Properties
  - CAS Number
    
    1400-61-9
    
    Molecular Formula
    
    C47H75NO17
    
    Molecular Weight
    
    926.09 g/mol (Nystatin A1)
    
    Other Properties
    
    Source: Streptomyces noursei and Streptomyces aureus and
    
    Storage
    
    -20°C ; Protect from light
    
    * For research use only
- Applications
  - Application Description
    
    Spectrum: Nystatin primarily targets fungi, yeasts and molds. It is active against Candida species, but in vitro, some species such as C. tropicalis can develop resistance. It is either fungistatic or fungicidal depending on the concentration of Nystatin, pH of the medium, and the nature of the infecting organism. It has a slightly narrower spectrum than Amphotericin B.
    
    Microbiology Applications: Nystatin is commonly used in clinical in vitro microbiological antimicrobial susceptibility tests (panels, discs, and MIC strips) against fungal isolates. Medical microbiologists use AST results to recommend treatment options. Representative effective ranges include:
    Candida albicans 0.78 µg/mL - 400 µg/mL
    Plant Biology Applications: Nystatin can be used in plant tissue culture to contol contamination.
    
    Eukaryotic Cell Culture Applications: Nystatin is commonly used to prevent contamination in cell culture.
    Quantum dots can be used to deliver and monitor biomolecules, but the study of the uptake mechanism is just beginning. Researchers found that Nystatin is a highly selective inhibitor of the lipid raft-dependent pathway and the uptake of SR9 is a lipid raft-dependent process, thus it can be a valuable compound for quantum dot research (Xu et al, 2010).