Name: Nisin Z
Price: 317.00 USD

- Overview
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    Background
    
    Nisin disrupts the cytoplasmic membrane via binding to the Lipid-II wall precursor to influence cell wall synthesis and us it as a docking molecule to form short-lived pores (2-2-5 nm) that are only stable for seconds, but can change the membrane potential, allowing an influx of ions, leading to cell death. In studies with HNSCC cells, researchers found Nisin acts through the cation transport regulator homolog 1 (CHAC1) and through a CHAC1-independent influx of extracellular calcium.
- Properties
  - CAS Number
    
    137061-46-2
    
    Molecular Formula
    
    C141H229N41O38S7
    
    Molecular Weight
    
    3331.05
    
    Appearance
    
    Light brown to milky white fine powder
    
    Solubility
    
    Soluble in water at neutral pH and above
    
    Other Properties
    
    Source: Lactococcus lactis
    Heavy Metals: ≤ 10 mg/kg
    Purity Level: ≥95% (anhydrous basis)
    
    Storage
    
    ≤30°C
    
    * For research use only
- Applications
  - Application Description
    
    Spectrum: Purified Nisin Z was found to have antibacterial activity against both Gram-positive and Gram-negative bacteria
    
    Microbiology Applications: Nisin is derived from culture on natural substrates such as milk or dextrose, since the chemical synthesis of this peptide is not cost-effective for industrial and large-scale applications. It can be used in food safety as a food additive to prevent spoilage. Nisin is used as a selective agent in microbiological media to isolate Gram-negative bacteria, yeast, and mold.
    
    Eukaryotic Cell Culture Applications: In vitro studies have shown that Nisin can modulate the innate immune system by causing the secretion of chemokines and suppressing LPS stimulated cytokines.
    
    Cancer Applications: Nisin Z induces selective toxicity and apoptotic cell death in cultured melanoma cells. The anti-melanoma potential of Nisin Z was evaluated in vitro. It induced selective toxicity and negatively affected the energy metabolism (glycolysis, mitochondrial respiration), increased ROS and caused apoptosis. It can also decrease the invasion and proliferation of melamona cells (Lewies et al, 2018).
    Nisin ZP, a naturally occurring variant, was found to have anti-cancer properties in studies with four human head and neck squamous cell carcinoma (HNSCC) cell lines: UM-SCC-17B (supraglottis/soft tissue-neck), UM-SCC-14A (floor of mouth), oral SCC cell lines from tongue (HSC-3 and OSCC-3). Nisin ZP induced apoptosis via a calpain-dependent pathway (Kamarajan et al, 2015).
    Nisin was found to have anti-cancer properties in studies with human head and neck squamous cell carcinoma (HNSCC), inducing preferential apoptosis and cell cycle arrest, and reducing cell proliferation. It can also reduce tumorigenesis in vivo. It exerts these effects through activation of CHAC1, a cation transport regulator, increased calcium influx, and induction of cell cycle arrest (Joo et al, 2012)
    The effect of Nisin on breast cancer cells was evaluated in vitro to study its cytotoxicity/proliferation using the MTT assay with the cell line MCF-7. It had a high and selective cytotoxicity with an IC50 value of 5 µM suggesting its potential use in breast cancer (Avand et al, 2018).
    The effect of Nisin on colon cancer was evaluated with colon cancer cells (SW480) and apoptostic effect using MTT assay and real-time PCR to find that it provokes the intrinsic pathway of apoptosis and increases bax/bcl-2 ratio in cancer cells (Ahmadi et al, 2017).