Nisin ReadyMade™ Solution

- Overview
  - Please contact us at for specific academic pricing.
    
    Background
    
    Nisin binds to lipid II which is a membrane-bound advanced intermediate involved in cell wall biosynthesis. It sequesters lipid II from its functional location. The nisin-lipid II complex leads to formation of pores in the bacterial membrane leading to bacterial cell death.
- Properties
  - CAS Number
    
    1414-45-5
    
    Molecular Formula
    
    C143H230N42O37S7
    
    Other Properties
    
    Source: Lactococcus lactis
    
    Storage
    
    -20°C
    
    * For research use only
- Applications
  - Application Description
    
    Spectrum: Nisin is effective against Gram-positive bacteria, but has little or no effect on Gram-negative bacteria, yeasts, or fungi.
    
    Microbiology Applications: Nisin can interact with the SARS-CoV-2 (2019 nCoV) spike protein receptor human antiotensin-converting enzyme 2 (hACE2) as found by in-silico homology modeling and docking of all 8 Nisin variants. Among the Nisin variants, Nisin Z and Nisin H showed significant binding affinity.
    The spike protein on the outer surface of SARS-CoV-2 virus is a recognition factor for its attachment and entry to the host cells. In humans, the receptor binding domain (RBD) of the spike protein binds to hACE2.
    Small peptides are suitable for disrupting protein-protein interactions (PPI) and have reduced immunogenicity, thus they are ideal candidates for novel therapeutics. The currently used peptides for COVID-19 are all synthetic and costly.
    
    Eukaryotic Cell Culture Applications: The influence of Nisin was evaluated on the proliferation and apoptosis of an astrocytoma cell line (SW1088). Cell death and apoptosis were significantly increased following Nisin treatment. This is a promising feature of Nisin for anti-cancer therapy.
    Diabetic foot ulcers (DFUs) are major complications of Diabetes mellitus. They are often recalcitrant to conventional antibiotics and alternatives are needed. The 3D collagen model used Nisin in combination with Pexiganan to control co-cultures of S. aureus and P. aeruginosa using a guar-gum delivery sysem (dual-AMP biogel) to eradicate S. aureus from the model (Gomes et al, 2020).