Iodoacetyl-LC-Biotin

- Overview
  - Please contact us at for specific academic pricing.
    
    Background
    
    Iodoacetyl-LC-Biotin (N-iodoacetyl-N-biotinylhexylenediamine), a sulfhydryl-reactive biotinylation agent, is a water-insoluble reagent that requires the dissolution of suitable solvents, including dimethyl sulfoxide (DMSO) and dimethylformamide (DMF), prior to the addition into aqueous reactions. Iodoacetyl-LC-Biotin consists of a bicyclic biotin rings structure, a 1,6-diaminohexane spacer group attached to the valeric acid side chain of biotin and an iodoacetyl group at the end of the spacer providing sulfhydryl reactivity. The iodoacetyl group at the end of Iodoacetyl-LC-Biotin is able to react with sulfhydryl groups on proteins and other molecules forming a nonreversible bond. The long spacer arm of Iodoacetyl-LC-Biotin enables the modified molecule to better bind to the avidin or streptavidin probes.
    Reference
    Bioconjugate Techniques , 2nd ed. By Greg T.Hermanson (Pierce Biotechnology, Thermo Fisher Scientific, Rockford, IL). Academic Press (an imprint of Elsevier): London, Amsterdam, Burlington, San Diego . 2008. ISBN 978-0-12-370501-3.
- Properties
  - Categories
    
    Sulfhydryl-reactive biotinylation reagent, iodoacetyl-activated
    
    Alternative Name
    
    N-Biotinyl-N'-(iodoacetyl)-1,6-hexanediamine,Iaa-biotin
    
    CAS Number
    
    93285-75-7
    
    Molecular Formula
    
    C18H31IN4O3S
    
    Molecular Weight
    
    510.43
    
    Appearance
    
    A solid
    
    Purity
    
    98.00%
    
    Solubility
    
    ≥51 mg/mL in DMSO with gentle warming; insoluble in EtOH; insoluble in H2O
    
    Storage
    
    Store at -20°C
    
    SMILES
    
    C1C2C(C(S1)CCCCC(=O)NCCCCCCNC(=O)CI)NC(=O)N2
    
    * For Research Use Only