NHS-Biotin

- Overview
  - Please contact us at for specific academic pricing.
    
    Background
    
    NHS-Biotin (C14H18O5N3S) is N-hydroxysuccinimido biotin. It is the shortest of three similar EZ-Link NHS-Biotin Reagents that enable simple and efficient biotinylation of antibodies, proteins and any other primary amine-containing biomolecules in solution. NHS-Biotin offers researchers the possibility of optimizing labeling and detection experiments where steric hindrance of biotin binding is an important factor. Because it is uncharged and contain simple alkyl-chain spacer arms, NHS-Biotin compound is membrane-permeable and useful for intracellular labeling.
    NHS-Biotin is the most popular type of biotinylation reagent. NHS-activated biotins react efficiently with primary amino groups (-NH2) in alkaline buffers to form stable amide bonds. Proteins (e.g., antibodies) typically have several primary amines that are available as targets for labeling, including the side chain of lysine (K) residues and the N-terminus of each polypeptide.
    
    Fouassier, L., Yun, C. C., Fitz, J. G. and Doctor, R. B. (2000). Evidence for Ezrin-Radixin-Moesin-binding Phosphoprotein 50 (EBP50) Self-association through PDZ-PDZ Interactions. J. Biol. Chem. 275, 25039-25045.
    Nunomura, W., Takakuwa, Y., Parra, M., Conboy, J.G., & Mohandas, N. (2000) Regulation of protein 4.1R, p55 and glycophorin C ternary complex in human erythrocyte membrane. J. Biol. Chem. 275(32): 24540-24546.
    Chiu, N.H. & T.K. Christopoulos. 1999. Two-site expression immunoassay using a firefly luciferase-coding DNA label. Clin. Chem. 45: 1954–1959..
- Properties
  - Categories
    
    Amine-reactive biotinyltation reagent
    
    Alternative Name
    
    Biotin N-hydroxysuccinimide ester, Biotin-OSU, Biotin NHS, Biotin SE
    
    CAS Number
    
    35013-72-0
    
    Molecular Formula
    
    C14H19N3SO5
    
    Molecular Weight
    
    341.38
    
    Appearance
    
    A solid
    
    Purity
    
    98.00%
    
    Solubility
    
    insoluble in EtOH; insoluble in H2O; ≥17.07 mg/mL in DMSO
    
    Storage
    
    Desiccate at -20°C
    
    SMILES
    
    C1CC(=O)N(C1=O)OC(=O)CCCCC2C3C(CS2)NC(=O)N3
    
    * For Research Use Only
- Reference
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    3. Wang P, Tseng KF, et al. "The Central Stalk Determines the Motility of Mitotic Kinesin-14 Homodimers." Curr Biol. 2018 Jul 23;28(14):2302-2308.e3. PMID:30017487
    4. Khan M, Schuster S, Zharnikov M. "Chemical derivatization and biofunctionalization of hydrogel nanomembranes for potential biomedical and biosensor applications." Phys Chem Chem Phys. 2016 Apr 28;18(17):12035-42. PMID:27067511
    5. Michael J. Bond,Avijeet S. Chopra,et al. "Characterization and Target Identification of AK301: A Novel Mitotic Arrest Agent." University of Connecticut.2016 4 29.
    6. Tomlinson, J. E., et al. "Survival Time of Cross‐Match Incompatible Red Blood Cells in Adult Horses." Journal of Veterinary Internal Medicine (2015). PMID:26478135