myo-Inositol Assay Kit

- Overview
  - The myo-Inositol Assay Kit is a reliable and accurate enzymatic UV-method for the specific measurement and analysis of myo-inositol in animal feeds, foods and various other materials.
    
    Phytic acid content of samples with very low levels of free myo-inositol can also be determined using K-INOSL. This can be achieved by measuring the amount of myo-inositol released after de-phosphorylation of phytic acid with the enzymes phytase and alkaline phosphatase, as used with the Phytic Acid/Total Phosphorus Assay Kit (K-PHYT).
    
    Not suitable for the determination of myo-inositol in baby formula.
    
    Note for Content: The number of manual tests per kit can be doubled if all volumes are halved. This can be readily accommodated using the MegaQuantTM Wave Spectrophotometer (D-MQWAVE).
    
    Please contact us at for specific academic pricing.
- Properties
  - Details
    
    Limit of Detection: 0.8mg/L
    
    Storage
    
    Short term stability: 2-8°C
    Long term stability: See individual component labels
    
    Stability
    
    > 2 years under recommended storage conditions
    
    * For research use only.
- Applications
  - Application Description
    
    Animal feeds, food and other materials.
- Reference
  - 1.Inositol in Disease and Development: Roles of Catabolism via myo-Inositol Oxygenase in Drosophila melanogaster. Contreras, A., Jones, M. K., Eldon, E. D. & Klig, L. S. (2023). International Journal of Molecular Sciences, 24(4), 4185.
    2.Myo-Inositol Supplementation in Suckling Rats Protects against Adverse Programming Outcomes on Hypothalamic Structure Caused by Mild Gestational Calorie Restriction, Partially Comparable to Leptin Effects. Castillo, P., Palou, M., Yau-Qiu, Z. X., Rodríguez, A. M., Palou, A. & Picó, C. (2021). Nutrients, 13(9), 3257.
    3.Deletion of Voltage-Dependent Anion Channel 1 knocks mitochondria down triggering metabolic rewiring in yeast. Magri, A., Di Rosa, M. C., Orlandi, I., Guarino, F., Reina, S., Guarnaccia, M., Morello, G., Spampinato, A., Cavallaro, S., Messina, A., Vai, M. & De Pinto, V. (2020). Cellular and Molecular Life Sciences, 77(16), 3195-3213.
    4.Impaired GCR1 transcription resulted in defective inositol levels, vacuolar structure and autophagy in Saccharomyces cerevisiae. Ravi, C., Gowsalya, R. & Nachiappan, V. (2019). Current Genetics, 65(4), 995-1014.
    5.The myo‐inositol converting pathway via glucuronic acid does not contribute to ascorbic acid synthesis in Arabidopsis. Ivanov Kavkova, E., Blöchl, C. & Tenhaken, R. (2018). Plant Biology, In Press.
    6.The myo-inositol/proton symporter IolT1 contributes to D-xylose uptake in Corynebacterium glutamicum. Brüsseler, C., Radek, A., Tenhaef, N., Krumbach, K., Noack, S. & Marienhagen, J. (2017). Bioresource Technology, 249, 953-961.
    7.L-Lysine production independent of the oxidative pentose phosphate pathway by Corynebacterium glutamicum with the Streptococcus mutans gapN gene. Takeno, S., Hori, K., Ohtani, S., Mimura, A., Mitsuhashi, S. & Ikeda, M. (2016). Metabolic engineering, 37, 1-10.
    8.Regulation of myo-inositol biosynthesis by p53-ISYNA1 pathway. Koguchi, T., Tanikawa, C., Mori, J., Kojima, Y. & Matsuda, K. (2016). International Journal of Oncology, 48(6), 2415-2424.

MSDS

myo-Inositol Assay Kit

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