Prime-Fect In Vivo - Primary Cell Transfection Reagent for siRNA and pDNA Delivery

Benefits
• High transfection efficiency in the presence of serum.
• Effective delivery of pDNA molecules via a simple protocol that is ideal for scale-up and automation.
• Minimal toxicity compared to other commercial reagents, reducing adverse effects on highly sensitive human cells.
• Non-integrating transfection reagent eliminates unwanted effects due to host genome integration.
• Possibility of using the same transfection reagent in animal models, leading to harmonized studies.

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Background

Prime-Fect is a highly effective transfection reagent for attachment-dependent primary cells. Prime-Fect is a synthetic amphiphilic polymer that is tailored primarily for DNA delivery to a wide range of primary cells. It is capable of undergoing multivalent interactions with polynucleotides and encapsulating co-incubated polynucleotides into 100-200 nm particles with a net positive charge. The complexation between the polynucleotides and the Prime-Fect occurs in aqueous buffers, obviating the need for organic solvents during preparation. Prime-Fect is a nonintegrating carrier, so that the genetic make-up of host cells is not altered after treatment with the transfection reagent. Prime-Fect has been tested and also found effective for siRNA delivery to different types of attachment dependent cells. As with all transfection reagents, formulations of Prime-Fect with DNA or RNA may need to be optimized for specific cell types and transfection conditions.