Mouse TNF-α T Cell ELISPOT Kit

Mouse TNF-α T Cell ELISPOT Kit

Catalog Number:
EK02494201UCY
Mfr. No.:
CT322-PR2/PR5
Price:
$754
  • Size:
    Quantity:
    Add to Cart:
      • Overview
        • The T cell ELISPOT assay is a simple and sensitive immunoassay for the detection of protein secreting T cells at the single cell level.

          Staining: Enzymatic
          Characteristics: PVDF

          Please contact us at for specific academic pricing.

      • Properties
        • Details
          • Coating antibodies
          • Biotinylated detection antibodies
          • Streptavidin-Horseradish Peroxidase
          • AEC coloring system
          • Blocking stock solution
          • Dilution buffer R
          • Tween-20
          • Detailed manual

          * For research use only.

      • Applications
        • Application Description
          The ELISPOT assay is one of the most sensitive assays to monitor ex vivo cellular immune responses at the single cell level. The assay can accurately detect secreted proteins, such as cytokines, released by T cells in response to an antigen. The cell suspensions used in the test can be from blood (PBMC), lymphoid, spleen, bone marrow or CNS tissue.
      • Reference
        • • Assessment of recombinant plasmid expressing fusion antigen Ag85B-Rv3425 in management of acute tuberculosis infection in mice.
          Huang, Huichang, et al. Exp Ther Med 2018 Mar;15: 3034-3039
          • Novel recombinant BCG coexpressing Ag85B, ESAT-6 and Rv2608 elicits significantly enhanced cellular immune and antibody responses in C57BL/6 mice.
          Lu, Y, et al. Scand. J. Immunol. 2012 Sep;76: 271-7
          • A novel method for efficient generation of antigen-specific effector T-cells using dendritic cells transduced with recombinant adeno-associated virus and p38 kinase blockade.
          Mirandola, Leonardo, et al. J Transl Med 2019 Dec 19;17: 424
          • Recombinant BCG coexpressing Ag85B, ESAT-6 and Rv3620c elicits specific Th1 immune responses in C57BL/6 mice.
          Yang, Enzhuo, et al. Microb. Pathog. 2014 Apr-May;69-70: 53-9

    Documents

    Note: If you don't receive our verification email, do the following:

    Copyright © Amerigo Scientific. All rights reserved.