Rat IL-4 T Cell ELISPOT Kit

Rat IL-4 T Cell ELISPOT Kit

Catalog Number:
EK02494203UCY
Mfr. No.:
CT081-PR2/PR5
Price:
$754
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      • Overview
        • The T cell ELISPOT assay is a simple and sensitive immunoassay for the detection of protein secreting T cells at the single cell level.

          Staining: Enzymatic
          Characteristics: PVDF

          Please contact us at for specific academic pricing.

      • Properties
        • Details
          • Coating antibodies
          • Biotinylated detection antibodies
          • Streptavidin-Horseradish Peroxidase
          • AEC coloring system
          • Blocking stock solution
          • Dilution buffer R
          • Tween-20
          • Detailed manual

          * For research use only.

      • Applications
        • Application Description
          The ELISPOT assay is one of the most sensitive assays to monitor ex vivo cellular immune responses at the single cell level. The assay can accurately detect secreted proteins, such as cytokines, released by T cells in response to an antigen. The cell suspensions used in the test can be from blood (PBMC), lymphoid, spleen, bone marrow or CNS tissue.
      • Reference
        • • Inhibition of collagen-induced arthritis by DNA vaccines encoding TCR Vbeta5.2 and TCR Vbeta8.2.
          Ge, Ping-ling, et al. Chin. Med. J. 2009 May 05;122: 1039-48
          • Mucosal and systemic immunization with targeted fusion anti-caries DNA plasmid in young rats.
          Liu, G X, et al. Vaccine 2009 May 14;27: 2940-7
          • Immune response induced in rodents by anti-CoVid19 plasmid DNA vaccine via pyro-drive jet injector inoculation.
          Nishikawa, Tomoyuki, et al. Immunol Med 2022 Dec;45(4): 251-264
          • Modulation of NKT cells and Th1/Th2 imbalance after alpha-GalCer treatment in progressive load-trained rats.
          Ru, Wang, et al. Int. J. Biol. Sci. 2009 May 07;5: 338-43

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