Mouse IL-4 T Cell ELISPOT Kit

Mouse IL-4 T Cell ELISPOT Kit

Catalog Number:
EK02494198UCY
Mfr. No.:
CT319-PR2/PR5
Price:
$754
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      • Overview
        • The T cell ELISPOT assay is a simple and sensitive immunoassay for the detection of protein secreting T cells at the single cell level.

          Staining: Enzymatic
          Characteristics: PVDF

          Please contact us at for specific academic pricing.

      • Properties
        • Details
          • Coating antibodies
          • Biotinylated detection antibodies
          • Streptavidin-Horseradish Peroxidase
          • AEC coloring system
          • Blocking stock solution
          • Dilution buffer R
          • Tween-20
          • Detailed manual

          * For research use only.

      • Applications
        • Application Description
          The ELISPOT assay is one of the most sensitive assays to monitor ex vivo cellular immune responses at the single cell level. The assay can accurately detect secreted proteins, such as cytokines, released by T cells in response to an antigen. The cell suspensions used in the test can be from blood (PBMC), lymphoid, spleen, bone marrow or CNS tissue.
      • Reference
        • • Vaccination of mice with recombinant baculovirus expressing spike or nucleocapsid protein of SARS-like coronavirus generates humoral and cellular immune responses.
          Bai, Bingke, et al. Mol. Immunol. 2008 Feb;45: 868-75
          • Protective effect of enterovirus‑71 (EV71) virus‑like particle vaccine against lethal EV71 infection in a neonatal mouse model.
          Cao, Lei, et al. Mol Med Rep 2015 Aug;12: 2473-80
          • Liposome-based polymer complex as a novel adjuvant: enhancement of specific antibody production and isotype switch.
          Chen, Chia-Hung, et al. Int J Nanomedicine 2012;7: 607-21
          • Removing N-terminal sequences in pre-S1 domain enhanced antibody and B-cell responses by an HBV large surface antigen DNA vaccine.
          Ge, Guohong, et al. PLoS ONE 2012;7: e41573
          • Induction of specific immune responses by severe acute respiratory syndrome coronavirus spike DNA vaccine with or without interleukin-2 immunization using different vaccination routes in mice.
          Hu, Hui, et al. Clin. Vaccine Immunol. 2007 Jul;14: 894-901
          • Characterization of the immune responses elicited by baculovirus-based vector vaccines against influenza virus hemagglutinin.
          Hu, Zhi-peng, et al. Acta Pharmacol. Sin. 2012 Jun;33: 783-90
          • Oral delivery of bi-autoantigens by bacterium-like particles (BLPs) against autoimmune diabetes in NOD mice.
          Mao, Ruifeng, et al. Drug Deliv 2023 Jan;30(1): 2173339
          • Intranasal immunization with recombinant Vaccinia virus Tiantan harboring Zaire Ebola virus gp elicited systemic and mucosal neutralizing antibody in mice.
          Xie, Lilan, et al. Vaccine 2019 May 31;37: 3335-3342
          • Administration with Vaccinia Virus Encoding Canine Parvovirus 2 Elicits Systemic Immune Responses in Mice and Dogs.
          Zhao, Wanbo, et al. Viral Immunol. 2020 Jul/Aug;33 (6): 434-443
          • A single immunization with HA DNA vaccine by electroporation induces early protection against H5N1 avian influenza virus challenge in mice.
          Zheng, Liyun, et al. BMC Infect. Dis. 2009 Feb 12;9: 17

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